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KMID : 0380620170490020123
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Korean Journal of Food Science and Technology
2017 Volume.49 No. 2 p.123 ~ p.131
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Monitoring of norovirus and indicator microorganisms from agricultural products and environmental samples in Korea
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Kang Ji-Hyun
Shim Hye-Mee
Kim Kwang-Yup
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Abstract
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Norovirus causes frequent epidemic viral gastroenteritis in Korea. The team for the control of noroviral foodborne outbreaks (NOROTECL) executed a project to trace the cause of norovirus contamination in agricultural products and environmental samples to reduce norovirus outbreaks in Korea. Between January and November in 2015, the contaminations by norovirus and indicator microorganisms such as coliforms, Escherichia coil and male specific coliphage (MSC) were examined in 80 agricultural products, 80 soil samples, 78 human feces samples, 3 animal feces samples, 80 agricultural water samples and 80 river water samples. Semi-nested PCR and DNA sequencing revealed 18 genogroup I and 3 genogroup II noroviruses in a total of 18 samples. These noroviruses were validated by real-time (RT)-PCR analysis. For indicator microorganisms, coliform and E. coli were respectively detected in agricultural products (68, 1%), soils (88, 7%), human feces (44, 12.8%), animal feces (67, 67%), agricultural waters (74, 30%) and river waters (96, 51%). The MSC results revealed 14 positive samples.
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KEYWORD
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norovirus, male-specific coliphage, semi-nested PCR, real-time RT-PCR
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